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Swirling the vial slowly afterward never shaking it supports full dissolution while protecting the peptides molecular structure, since vigorous agitation can degrade sensitive peptide bonds

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When considering the interaction between loss of pink-1 and metabolite addition, these mutant lines confirm RNAi results, suggesting epistatic regulation of metabolite activity by loss of pink-1 (Figures 4d and e) but not pdr-1, which appears to acts in parallel to metabolite toxicity

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